Microinjection ICSI is a fertilization method used in in vitro fertilization, which is made by injecting a single sperm from the father-to-be into a healthy egg taken from the mother-to-be. In the classical IVF treatment, the sperm and egg cell are brought face to face in the laboratory and the sperm cell is expected to fertilize the egg cell. Fertilization in classical IVF treatment is similar to fertilization in a normal pregnancy. The only difference is that it takes place in a laboratory setting.

In some cases, the sperm cell cannot fertilize the egg cell. If the sperm motility, number of sperm cells and morphology are not sufficient for fertilization of the egg, this method is used. The only difference between these two methods is the fertilization phase.

Microinjection ICSI  was first applied in Belgium in 1992. Previously, individuals with sperm count below 5 million/mL or with very low quality sperm analysis had little or no chance of having children. However, this method, which was developed with the development of technology and medical science, is a common and frequently preferred application today.

This method is applied by embryologists who are experts in their fields, using technological microscopes and micromanipulators. In this application, the sperms taken from the father-to-be are examined by magnifying 400 times, the healthiest and best quality sperm are determined and selected for this method. The embryologist holds the egg with the help of a thin glass pipette and takes a single sperm cell with a glass needle 7 times thinner than a human hair and injects it directly into the egg cell under the microscope. Procedures such as following and monitoring the patient after this fertilization process are the same as classical IVF procedures.

When fertilization is performed with this method, a success of around is achieved for each egg in which the sperm is injected. Although it is more troublesome than other methods, it is a reproductive technique that provides help for mothers and fathers who want to have children. Since a single sperm cell is sufficient in this method, even men with very low sperm count or severely infertile male patients have a chance to have a child.

To Whom Is Microinjection Application Applied?

• In patients with very low sperm numbers or in patients with azoospermia.
• In women with tubal obstruction.
• In women who produce antibodies against sperm cells.
• Couples who have undergone classical IVF treatment but have not achieved any results.
• In case the sperm cell is morphologically defective.
• In patients who will undergo preimplantation genetic diagnosis.
• In cases of infertility of unknown cause.
• In some selected patients to increase the chance of pregnancy.
• In patients with sperm obtained by micro TESE method.

Sperm Antibodies:

In some women’s bodies, substances called antibodies are secreted against sperm cells. These antibodies prevent the sperm cell from fertilizing the egg cell. For such cases, according to the amount and nature of the antibody, Microinjection ICSI treatment is preferable.

Tubal Occlusion:

It is a condition in which women have a blockage in their tubes for any reason. Normally, sperm cells entering the cervix after sexual intercourse reach the fallopian tubes and meet the ovulated egg. The fertilization phase takes place inside these tubes. However, if there is a blockage in the tubes for any reason, the fertilization process does not take place. Because, Microinjection ICSI treatment provides success in such couples.

What are the Advantages of Microinjection Method?

• Since the sperm is injected directly into the egg cell, fertilization is likely to occur with this method.
• Patients with low sperm count and quality can have children through this method.
• This application can be done with a single sperm obtained.
• The risk of anomaly in babies born with this method is not higher than other methods.

Difference Between Microinjection Method and Classical IVF Application

In the classical IVF application, a large number of sperm cells taken from the father-to-be are left around an egg cell taken from the mother-to-be. Pregnancy is expected to occur when any of these sperm fertilizes the egg.

Microinjection ICSI In the method, a single sperm cell is injected directly into the egg cell. Thus, reproductive cells are directly exposed to the fertilization process. The probability of fertilization and the occurrence of pregnancy is higher than conventional in vitro fertilization.

Chance of Fertilization in Microinjection Application

In this technique, sperm cells are injected directly into the egg using special equipment under a microscope. Generally, fertilization is expected to occur. However, in some cases, fertilization may not occur. Fertilization takes place; The quality of the egg cell depends on the quality of the sperm cell and the experience of the embryologists.

What is the Chance of Pregnancy with Microinjection Method?

With this method, the probability of pregnancy is higher than with the classical IVF method. Although it varies according to age, the chance of pregnancy in this method is between and 80. However, the chances of success vary from person to person. Factors such as age, ovarian reserve, sperm parameters and intrauterine status are variables that affect the probability of pregnancy.

Does Anomaly Risk Increase With Microinjection Application?

In the researches, Microinjection ICSI No higher risk was observed in terms of a different symptom, birth defect or genetic anomaly in babies born by the birth method than babies born from naturally occurring pregnancies. Chromosome analysis would be beneficial in couples with genetically at risk of anomaly and an anomaly in the family history. By performing genetic screening, the risk of anomalies will be reduced. For a healthy baby, genetic examination would be more appropriate.

How is Microinjection Technique Applied?

The best quality sperms in the semen sample taken from the father-to-be are determined and taken through a microscopic injector. This sperm cell taken is directly injected into the egg cell obtained from the expectant mother. Fertilization is then awaited. Before this application, the environment of the egg is cleaned. When the egg is in the metaphase-2 stage Microinjection ICSI With the fertilization method, the fertilization process is completed.

Importance of Single Embryo Transfer

Since single embryo transfer is performed in this treatment method, the rates of multiple pregnancies and complications related to these pregnancies have decreased. In multiple pregnancies; There are disease risks such as premature birth, death of the baby in the womb, early water coming, pregnancy-related diabetes and blood pressure diseases. These risks are avoided with a single embryo transfer.

Is There a Risk for Those Who Have Microinjection?

As with any medical procedure Microinjection ICSI There are some risks involved in the process. Some potential risks that may arise during the procedure are as follows:

• Egg cells may be damaged.
• After the fertilization stage, the embryo may not develop.
• In the first stages of treatment; There may be side effects such as headache, vaginal discharge, feeling of warmth, tenderness in the breasts.
• Ovarian hyperstimulation syndrome may rarely be experienced due to hormonal drugs.
• When more than one embryo is placed in the uterus, the twin baby rate is above . The risk of triplet babies is below %1. In multiple pregnancies, the risk of abortion, low birth weight and premature birth is high.

However, when this procedure is performed under appropriate laboratory conditions by expert embryologists, the risk is minimized. We, as IVOX hospital, provide you with services in this field with our expert and experienced embryologists. Therefore, you can safely choose us for this treatment protocol.

Sperm Selection in Microinjection Method

Selection of quality sperm is important to increase the chance of conception. Sperm cells with a good appearance; In terms of excess or deficiency in the number of chromosomes and the maturity of the sperm, Microinjection ICSI There may not be enough quality and healthy cells for the process. If there is any damage to the sperm cell, pregnancy can progress with abortion. Therefore, sperm selection, Microinjection ICSI important for its application.

Sperm cells taken from the father-to-be are examined at 400 times magnification, and sperm cells that do not pose a morphological problem are detected. Sperm cells that only have a smooth appearance do not provide information about the correctness or maturity of their DNA.

With the help of a special solution called sperm slow, sperm cells are distinguished. In this solution, mature sperm cells with a high percentage of normal chromosomes can be detected easily. In this way, quality embryos and healthy results are obtained.

Stages of Microinjection Treatment

We apply different treatment protocols according to the condition of our patients. Generally, the procedure begins with the patient’s decision to treat. The patient begins to take birth control pills with the first menstrual period. The purpose of using birth control pills; It is to prevent ovarian cyst, to have menstrual period on time and to start treatment easily in the next month. The birth control pill is used for 21 days. 10 days after these pills are finished, the new menstrual period begins.

• Stimulation of the ovaries

In order to stimulate the ovaries, injections containing hormones are started on the 3rd day of the menstrual period. This application is important to ensure the development of more than one egg cell in the ovaries. The duration of this application, which increases the chance of pregnancy, varies from person to person, and it takes 10-12 days on average. 6 days after starting this hormone therapy, antagonists are added to the treatment protocol. Antagonists are continued until the end of the treatment. Egg development is followed by ultrasound.

• collection of ovaries

When the egg cells reach a certain size, a different hormonal needle is used for the eggs. Mature eggs are selected for collection. Egg collection is performed 34-36 hours after this injection. It is of great importance that this hormonal injection is made on time.

• Fertilization of the ovaries

By injecting the egg into the sperm cell taken from the father-to-be. Microinjection ICSI method is applied. With the help of a device called a micromanipulator, each sperm cell is injected into an egg. This process is done after the outer surface of the eggs is cleaned. After this process, the eggs are removed to the incubator devices in containers containing special solutions. 12-15. Fertilization takes place at the end of the hour. The fertilization process is detected under the microscope. Whether the eggs are fertilized or not, Microinjection ICSI It is the most important step in the treatment. It can be detected one day after the egg collection stage. After detection, the result is reported to the patient. Thus, the time for embryo transfer is decided. When to transfer is decided depending on the quality and number of embryos.

• Embryo transfer

After fertilization, the embryo is formed. Embryo transfer is performed 48-72 hours after egg collection and sometimes 120 hours later. Embryos are placed into the uterus with the help of a thin plastic tubular catheter. This process requires precision and attention. Embryo transfer is a painless procedure. After the application of this procedure, which does not require anesthesia, you can be discharged after a half-hour rest period. Two weeks after this procedure, a pregnancy test is performed.

• pregnancy support

We give progesterone hormone to our patients after the embryo transfer process. Progesterone is a hormone that has a protective role in pregnancy and is actually produced by the body. If the patient has a progesterone deficiency, early abortion situations are prevented with the help of this support. If the patient is pregnant, progesterone support is continued until the 12th week of pregnancy.

Is Egg Collection Painful?

Eggs are taken directly from the ovarian follicles by transvaginal ultrasound aspiration under short-term general anesthesia. The procedure is a painless procedure.

How Long Does the Microinjection Stage Take?

Microinjection ICSI The second stage is the stage of injecting a sperm into each mature and large egg collected after ovarian stimulation. Although this process varies depending on the number of mature eggs, it takes 10-20 minutes on average. After this process, the eggs are removed to the incubator devices in a special solution. The next day, it is checked whether they are fertilized or not.

Will Ovarian Reserve Decrease As A Result Of This Treatment?

Stimulation of eggs for in vitro fertilization does not reduce ovarian reserve, does not cause depletion of ovarian reserve.

What is Incubator?

The incubator provides a stable and clean environment for embryo culture. It has the temperature, carbon dioxide and oxygen levels necessary for the embryos to survive and mimics the mother’s uterus.

Is the Microinjection Method Repeatable?

Microinjection ICSI It can be reapplied when the application is unsuccessful. This method has no limitations. However, since hormonal drugs are used during the treatment, estrogen hormone also increases depending on the stimulated eggs. This creates confusion in the body. For this reason, it would be more appropriate to start the treatment again after 2-3 menstrual periods in order for the body to collect itself.

Microinjection Process and Grafting Process Are Different

In the vaccination process, the sperm in the semen taken from the father-to-be are washed and sent to the uterus via a catheter. The success rate in grafting is between and 15.

Classic IVF treatment or this treatment protocol is started for couples who have been vaccinated 3 times but without success.

Microinjection is a Successful Treatment in Infertility

Infertility is a condition that prevents the birth of children naturally. In the presence of infertility problem, the classical in vitro fertilization method is not an adequate method to have a child. In the presence of infertility, this method provides success for pregnancy formation. At the same time, since quality sperm are used in this process, the chance of fertilization increases. Precise work done in expectant mothers, from egg collection to peeling and fertilization, increases the probability of success. For this reason, infertility is no longer a problem and infertility seen in one or both of the couples does not prevent having a child.

If you have an infertility problem, you can safely choose us for IVF treatment with this method.

Is Microinjection Effective on Vasectomy?

Vasectomy, one of the birth control methods, means the dysfunction of the sperm ducts of the man. A man who has had a previous vasectomy Microinjection ICSI method, they can have children. The sperm extracted from the male testicles are placed into the egg with this method.

Tests to be Done Before Treatment

Microinjection ICSI A treatment plan should be created based on the patient’s age, ultrasound results and hormone tests. It is important that both the male patient and the female patient be subjected to comprehensive tests and examinations and a detailed examination is made. Like this Microinjection ICSI Before starting drug treatment, necessary data are collected.

For woman:

• Systemic and local physiological examination: Gynecological examination, gynecological ultrasonography, detailed examination of the vagina, cervix and uterus.
• Hormone tests: FSH, LH, Prolactin, E2, TSH, Progesterone and AMH hormones are checked. It is important for us to examine the hormones so that we can see the ovarian functions in general.

For men:

• Systemic and local physiological examination.
• Semen analysis: Evaluation of sperm count and motility, comprehensive morphological evaluation and quality of sperm. If the sperm parameters are below the values determined by the World Health Organization, additional examinations are requested.

Microinjection Treatment Cost

The fee may vary according to the age of the patients, ovarian reserve and the desired tests and examinations. Before treatment, some tests and examinations are requested. A treatment protocol is created by requesting hormone tests and ultrasound examination for sperm analysis in male patients and ovarian reserve measurement in female patients. Therefore, the cost varies. We, as IVOX hospital, offer our services to you with an economical price policy.

Importance of Microinjection Process

High rate of fertilization, Microinjection ICSI This is one of the main reasons why the method is a very advantageous method. Especially considering the problems related to the number, motility and quality of sperm, Microinjection ICSI The importance of the method becomes much clearer. Because in the presence of these problems, the probability of fertilization with the classical in vitro fertilization method is low. Especially in the presence of conditions such as azoospermia, fertilization becomes almost impossible. While healthy sperms enter the egg themselves and perform the fertilization process, Microinjection ICSI With the choice of treatment, it helps the fertilization process.

Since this process can be achieved with a single sperm cell, the chance of fertilization increases by choosing the best quality sperm cell. In the presence of conditions such as azoospermia, if there is no sperm in the semen, there may be a situation such as the removal of a few sperm cells from the testis. By taking sperm cells with methods such as TESE Microinjection ICSI phase can be passed. In other words, even in the presence of conditions such as azoospermia, the chance of having a child continues.

This practice, which started in 1992, has helped infertile couples to bring children into the world. Assisted reproductive technology and fertility treatment by medical experts have been a hope for infertile couples. This application, which has become widespread all over the world, is a revolutionary treatment method especially for infertility problem. In recent years, this procedure is also preferred for women with low egg reserve. The most important reason why this treatment is preferred over the classical IVF treatment is the higher fertilization rate.

Get Microinjection Treatment Safely at IVOX Hospital

In cases where conventional IVF treatment is insufficient, couples Microinjection ICSI can apply for treatment. Couples who want to increase the probability of success can also choose. Microinjection ICSIIt is a method that is preferred for many reasons and that has been frequently used recently.

Microinjection ICSI The procedure is a very sensitive treatment method that requires microsurgery. If this micro-operation is not performed by experienced embryologists in optimum laboratory conditions, there may be situations such as fertilization failure, degeneration of eggs to the point where they lose their viability, failure of embryo development, and problems in the quality of the embryo.

Microinjection ICSI Only mature and healthy eggs are used in the process. One per egg; The fast, motile, morphologically most straight-looking sperms are detected and taken with the help of a pipette and transferred into a solution that slows the movement of the sperm. In this solution, the sperm tail is broken and the sperm cell with the broken tail is placed in the cytoplasm of the egg. When the process is finished, the eggs are transferred into fresh culture broth and left to incubate until fertilization control.

It would be the right choice to receive such a detailed, sophisticated and comprehensive application in a reliable and quality hospital. The presence of health institutions and specialist physicians with all technical equipment increases the chance of success. We, as IVOX hospital, provide you with services in the field of health with our expert and experienced staff, our innovative attitude, our technological devices and equipment. You can choose us with confidence for a quality and healthy service process.

To have detailed information about our services or to make an appointment, you can contact us from the contact information.